产品说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone RH95 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Glutamate receptor 1 (GluR1). It targets an epitope within the N-terminal region.
免疫原
Thyroglobulin-conjugated linear peptide from the N-terminal region of rat Glutamate receptor 1.
应用
Anti-GluR1, clone RH95 ZooMAb, Cat. No. ZMS1007, is a recombinant Mouse monoclonal antibody that specifically targets GluR1 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected GluR1 in Mouse Brain tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GluR1 in rat brain tissue sections.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected GluR1 in rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GluR1 in rat E18 cortical cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Glutamate receptor 1 (UniProt: P19490; also known as GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, Glutamate receptor ionotropic, AMPA 1, GluA1) is encoded by the Gria1 (also known as Glur1) gene (Gene ID: 50592) in rat. Four different AMPA receptors have been described and are designated as GluR1, GluR2, GluR3, and GluR4. These receptors mediate fast excitatory neurotransmission in the brain and are involved in multiple brain functions, including cognition, movement, learning, and memory. Each of the AMPA receptor subunit can exist in either ′flip′ or ′flop′ form and the efficacy of glutamate is believed to be higher at the ′flip′ form. The binding site on this receptor is formed by the N-terminal tail and the extracellular loop between transmembrane domains three and four. Upon agonist binding, these two loops move towards each other, which opens the pore.Glu-R1 is a multi-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that contains to extracellular domains, four transmembrane domains, and two cytoplasmic domains. It is widely expressed throughout the nervous system and can exist in homotetrameric or heterotetrameric form. Tetramers may be formed by the dimerization of dimers. GluR-1 has several phosphorylation sites on the intracellular carboxy terminus. Many of these sites play a role in receptor regulation and synaptic plasticity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, HK., et al. (2010). J. Physiol. 103(1); 479-489).
外形
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
质量水平 | 200 |
生物来源 | mouse |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | RH95, monoclonal recombinant monoclonal |
产品线 | ZooMAb® learn more |
形式 | lyophilized |
分子量 | calculated mol wt 101.58 kDa observed mol wt ~110 kDa |
species reactivity | mouse, rat |
包装 | antibody small pack of 25 μL |
环保替代产品特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | immunocytochemistry: suitable immunofluorescence: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
同位素/亚型 | IgG2a |
环保替代产品分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich