产品说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 2O18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Bcl-2-like protein 11 (BIM) protein. It targets an epitope within 19 amino acids from the N-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human BIM.
应用
Anti-BIM, clone 2O18 ZooMAb, Cat. No. ZRB1375, a recombinant Rabbit monoclonal antibody that targets BIM and is tested in Affinity Binding, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected BIM in mouse spleen tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected BIM in human breast cancer and human lung cancer tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected BIM in RAW264.7 cells.
Affinity Binding Assay: A representative lot of this antibody bound BIM peptide with a KD value of 3.9 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
目标描述
Bcl-2-like protein 11 (UniProt: O43521; also known as Bcl2-L-11, Bcl2-interacting mediator of cell death, BIM) is encoded by the BCL2L11 (also known as BIM) gene (Gene ID: 10018) in human. BIM is a pro-apoptotic member of the Bcl-2 family that plays an important role in Bax/Bak-mediated cytochrome c release and apoptosis in response to various intrinsic stimuli. Like other members of the Bcl-2 family, BIM possesses a hydrophobic C-terminus that helps it to localize to intracytoplasmic membranes. In normal healthy cells BIM is localized to the microtubule-associated dynein motor complex in association with the LC8 dynein light chain; however, when subjected to apoptosis stimuli, it dissociates from LC8 and translocates to the mitochondria where it interacts with Bcl-2 to block its anti-apoptotic activity. Its N-terminal plays a role keeping it bound to the cytoskeleton and removal of that allows it to translocate to mitochondria. BIM contains a BH3 motif (aa 148-162) that is essential for its interaction with Bcl-2 proteins. BIM can undergo phosphorylation at serine 69 by MAPK1/MAPK3 that leads to its interaction with tripartite motif containing 2 (TRIM2), an E3 ubiquitin ligase, and polyubiquitination, followed by proteasomal degradation. BIM-deficient mice display higher numbers of B cells, CD4 and CD8 single-positive T cells, macrophages and granulocytes in their periphery. BIM is also reported to be essential for the deletion of auto-reactive B and T cells. Lymphocytes lacking BIM are refractory to several stimuli, including cytokine deprivation, deregulated calcium ion flux. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: O Connor, L., et al (1998). EMBO J. 17(2); 384-395; Gogada, R., et al. (2013). J. Biol. Chem. 288(1); 368-381).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
生物来源 | rabbit (recombinant) |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 2O18, monoclonal recombinant monoclonal |
产品线 | ZooMAb® learn more |
形式 | lyophilized |
分子量 | calculated mol wt 22.17 kDa observed mol wt ~22 kDa |
species reactivity | human, rat, mouse |
包装 | pkg of 25 μL |
环保替代产品特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG |
环保替代产品分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
Sigma-Aldrich