产品说明
一般描述
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特异性
Clone 1G2 is a Rabbit recombinant monoclonal antibody that specifically detects Cathepsin L. It targets an epitope within 14 amino acids from the C-terminal half. It detects procathepsin L, mature Cathepsin L, and Cathepsin L heavy chain.
免疫原
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of human Cathepsin L.
应用
Quality Control Testing
Evaluated by Western Blotting in PC12 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Cathepsin L1 in PC12 cell lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Cathepsin L1 in A549 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound Cathepsin L1 peptide with a KD of 4.1 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cathepsin L1 in A549 cells
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cathepsin L1 in human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Procathepsin L (UniProt: P07711; also known as EC:3.4.22.15, Cathepsin L, Major excreted protein, MEP) is encoded by the CTSL (also known as CTSL1) gene (Gene ID: 1514) in human. Cathepsin L is a lysosomal protease that is synthesized as a procathepsin with a signal peptide (aa 1-17) and two propeptides (aa 18-113 activation peptide; and 289-291) that are cleaved off to produce mature active enzyme. After the removal of these peptides, cathepsin can exist in three forms: cathepsin L (aa 114-333), cathepsin L heavy chain (aa 114-288) and cathepsin L light chain (aa 292-333). The heavy and light chains are connected by disulfide bonds. Active cathepsin L is a thiol protease with endopeptidase activity and preferentially cleaves peptide bonds with aromatic residues in P2 position and hydrophobic residues in P3 position. It is catalytically active in the pH range 3 to 6.5. It participates in overall degradation of proteins in lysosomes and plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. It is also Involved in the solubilization of cross-linked thyroglobulin and in subsequent release of thyroxine (T4) by limited proteolysis of thyroglobulin in the thyroid follicle lumen. Cathepsin L has been implicated in several pathological conditions, including invasion and tumor metastasis, atherosclerosis, inflammation, and degradation of extracellular matrix that is reported to be an important process in entry of SARS-CoV-2 into host cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gomes, CP., et al. (2020). Front. Cell. Infect. Microbiol. 10; 589505; Turk, V., et. al. (2012). Biochim. Biophys. Acta. 1824(1); 68-88).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
质量水平 | 200 |
生物来源 | rabbit |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 1G2, recombinant monoclonal |
描述 | recombinant, expressed in HEK 293 cells |
产品线 | ZooMAb® learn more |
形式 | lyophilized |
分子量 | calculated mol wt 37.56 kDa observed mol wt ~25, 32, 37 kDa |
纯化方式 | using Protein A |
species reactivity | rat, human |
species reactivity (predicted by homology) | monkey |
包装 | antibody small pack of 25 μL |
环保替代产品特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
同位素/亚型 | IgG |
表位序列 | C-terminal half |
Protein ID登记号 | NP_001903.1 |
UniProt登记号 | P07711 |
环保替代产品分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich