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位置:首页 > 品牌 > Sigma-Aldrich > Anti-CGRP-1 Antibody, clone 2E18 ZooMAb® Rabbit Monoclonal

Anti-CGRP-1 Antibody, clone 2E18 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1267
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-CGRP-1 Antibody, clone 2E18 ZooMAb® Rabbit Monoclonal
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

特异性

Clone 2E18 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Calcitonin gene-related peptide 1. It targets an epitope within 22 amino acids from the active form of CGRP1.

免疫原

KLH-conjugated linear peptide corresponding to 22 amino acids from human active Calcitonin gene-related peptide 1 (C-terminal region of the uncleaved peptide)

应用

Quality Control Testing
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Calcitonin Gene-Related Peptide 1 (CGRP-1) in MCF-7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CGRP-1 in PC12 and K562 cells and in Human and mouse liver lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CGRP-1 in human cerebral cortex and human DRG tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound CGRP-1 peptide with a KD of 1.9 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CGRP-1 in MCF7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CGRP-1, clone 2E18 ZooMAb®, Cat. No. ZRB1267, is a recombinant Rabbit monoclonal antibody that specifically targets CGRP-1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Calcitonin gene-related peptide 1 (UniProt: P06881; also known as Alpha-type CGRP, Calcitonin gene-related peptide I, CGRP-I) is encoded by the CALCA (also known as CALC1) gene (Gene ID: 746) in human. CGRP, a 37 amino acid neuropeptide, is a highly potent and long-acting vasodilator with protective effects in the cardiovascular system. Two forms of CGRP have been described -CGRP-I and CGRP-II (also known as aCGRP and bCGRP) that are synthesized from two distinct genes. CGRP I is the primary neuronal form that is found in the CNS and in the peripheral nervous system and displays higher immunoreactivity than CGRP II. CGRP I is synthesized with a signal peptide (aa 1-25) and two peptides (aa 26-80 and 125-128) that are cleaved off to generate biologically active form (aa 83-119). Following synthesis, it is stored in large, dense-core vesicles within the sensory nerve terminal and is released following neuronal depolarization via calcium-dependent exocytosis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Russell, FA., et al. (2014). Physiol. Rev. 94(4): 1099-1142).

外形

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit
质量水平200
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆2E18, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt ~ 13.90 kDa
observed mol wt ~ 14 kDa
species reactivityhuman, mouse, rat
包装antibody small pack of 25 μL
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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