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位置:首页 > 品牌 > Sigma-Aldrich > Anti-FADD Antibody, clone 11N7, ZooMAb® Rabbit Monoclonal

Anti-FADD Antibody, clone 11N7, ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB06711
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2001.06 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-FADD Antibody, clone 11N7, ZooMAb® Rabbit Monoclonal
FAS-associated death domain protein
Mediator of receptor induced toxicity
Protein FADD
FAS-associating death domain-containing protein
Growth-inhibiting gene 3 protein
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 11N7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human FAS-associated death domain protein (FADD).

免疫原

His-tagged full-length recombinant human FAS-associated death domain protein (FADD).

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected FADD in MCF7 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FADD in human kidney tissue sections.
RNAi/siRNA/Gene Knockdown Analysis: A 1:250 dilution from a representative lot detected FADD lysates from Wild type HeLa cells, but not in FADD knockout HeLa cells.
Anti-FADD, clone 11N7, ZooMAb, Cat. No. Cat. No. ZRB06711, is a recombinant Rabbit Monoclonal antibody that detects human FAS-associated death domain and has been tested for use in Immunohistochemistry (Paraffin), RNAi/siRNA/Gene Knockdown, and Western Blotting.

目标描述

FAS-associated death domain protein (UniProt: Q13158; also known as FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD) is encoded by the FADD (also known as MORT1, GIG3) gene (Gene ID: 8772) in human. FADD is an apoptotic adaptor molecule that bridges death receptor signaling to the caspase cascade. It is expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. FADD contains a death domain at its C-terminus and a death effector domain (DED) at its N-terminal. The death domain is responsible for FADD binding to the activated trimerized Fas receptor. FADD recruits procaspase-8 through its N-terminal DED, which is then activated following the cleavage of pro domain. The death inducing signaling complex (DISC) formed by Fas receptor, FADD, and caspase-8 is essential in triggering apoptotic events in cells. Human FADD is phosphorylated mainly at Ser194. Defects in FADD gene have been linked to recurrent infections, hepatic dysfunction, and cardiovascular malformations. These subjects display high circulating levels of CD4(-) and CD8(-) cells and elevated levels of IL-10. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆11N7, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 23.28 kDa
observed mol wt ~26 kDa
species reactivityhuman
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)gene knockdown: suitable using 1:250
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100
western blot: suitable using 1:1,000
同位素/亚型IgG
UniProt登记号Q13158
环保替代产品分类Aligned
运输ambient
储存温度2-8℃
Gene Informationhuman ... FADD;MORT1;GIG3(8772)

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1

Sigma-Aldrich

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