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位置:首页 > 品牌 > Sigma-Aldrich > Anti-FOXO3A Antibody, clone 1G18 ZooMAb® Rabbit Monoclonal

Anti-FOXO3A Antibody, clone 1G18 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1597
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-FOXO3A Antibody, clone 1G18 ZooMAb® Rabbit Monoclonal
Forkhead box protein O3;AF6q21 protein;Forkhead in rhabdomyosarcoma-like
产品介绍:

产品说明

一般描述

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特异性

Clone 1G18 is a Rabbit recombinant monoclonal antibody that specifically detects Forkhead box protein O3 (FOXO3A). It targets an epitope within 20 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human Forkhead box protein O3 (FOXO3A).

应用

Quality Control Testing
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FOXO3A in MCF-7 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected FOXO3A in SH-SY5Y cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected FOXO3A in human colon tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot from a representative lot detected FOXO3A in SH-SY5Y cells.
Affinity Binding Assay: A representative lot of this antibody bound FOXO3A with a KD of 3.0 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Forkhead box protein O3 (UniProt: O43524; also known as AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1) is encoded by the FOXO3 (also known as FKHRL1, FOXO3A) gene (Gene ID: 2309) in human. Forkhead box protein O3 is a transcriptional activator that triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. It recognizes and binds to the DNA sequence 5′-[AG]TAAA[TC]A-3′. Its DNA binding domain located in amino acids 157-251. The nuclear localization signal is localized in amino acids 242-259. In the cytoplasm it is present in an inactive state and it translocates to the nucleus upon oxidative stress and in the absence of survival factors. In response to metabolic stress, it translocates into the mitochondria where it promotes mtDNA transcription. In the presence of survival factors such as IGF-1, it is phosphorylated on threonine 32 and serine 253 by AKT1/PKB. The phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes its translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. FOXO3 deacetylation by SIRT1 or SIRT2 stimulates its interaction SKP2 and facilitates SCF(SKP2)-mediated FOXO3 ubiquitination and proteasomal degradation. A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Peserica, A., et al. (2013). Cell Mol. Life Sci. 70(11); 2015-2029; Brunet, A., et al. (1999). Cell. 96(6); 857-868).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源rabbit
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1G18, recombinant monoclonal
描述recombinant, expressed in HEK 293 cells
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 71.28 and 48.41 kDa
observed mol wt ~100 kDa
纯化方式using Protein A
species reactivityhuman
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
表位序列C-terminal half
Protein ID登记号NP_001446
UniProt登记号O43524
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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