您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Anti-GAP43 Antibody, clone 1D6 , ZooMAb® Rabbit Monoclonal

Anti-GAP43 Antibody, clone 1D6 , ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1220
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-GAP43 Antibody, clone 1D6 , ZooMAb® Rabbit Monoclonal
Axonal membrane protein GAP-43
Growth-associated protein 43
Neural phosphoprotein B-50
Neuromodulin
pp46
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 1D6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Growth-Associated Protein 43 (GAP43).

免疫原

GST/His-tagged recombinant fragment corresponding to the first 126 amino acids from the N-terminal half of human Growth-Associated Protein 43 (GAP43).

应用

Anti-GAP43, clone 1D6 ZooMAb, Cat. No. ZRB1220, is a highly specific rabbit recombinant monoclonal antibody that targets Growth-associated protein 43 (GAP43) and has been tested for use Immunocytochemistry, Immunohistochemistry (Paraffin). And Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representiatve lot detected GAP43 in human and mouse cerebral cortex tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representiatve lot detected GAP43 in SH-SY5Y cells.
Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.

目标描述

Neuromodulin (UniProt: P17677; also known as Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46) is encoded by the GAP43 gene (Gene ID: 2596) in human. GAP43 is a peripheral membrane protein that is associated with nerve growth. It serves as a common marker of differentiating neurons. It is a major component of the motile "growth cones" that form the tips of elongating axons and plays a role in axonal and dendritic filopodia induction. It is expressed at high levels in neuronal growth cones during development and axonal regeneration. However, it is not fond in dendritic cones. In adult brain, GAP43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. GAP43 contains an N-terminal lipid binding IQ domain (aa 31-60) that also binds calmodulin. GAP43 is reported to bind calmodulin at low calcium levels and then releases it when calcium levels increase indicating that calmodulin may serve as a negative regulator of GAP43 during periods of low neuronal activity. Protein kinase C-mediated phosphorylation serine 41 is reported to inhibit this interaction and stabilizes actin filaments and promotes growth cone extension. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.(Ref.: Neve, RL et al., (1998). J. Neurosci. 18(19); 7757-7767).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1D6, recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 24.8 kDa
observed mol wt ~47 kDa
species reactivityhuman
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品