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位置:首页 > 品牌 > Sigma-Aldrich > Anti-NG2 Antibody, clone 1L2, ZooMAb® Rabbit Monoclonal

Anti-NG2 Antibody, clone 1L2, ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB5320
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
Anti-NG2 Antibody, clone 1L2, ZooMAb® Rabbit Monoclonal
Chondroitin sulfate proteoglycan 4
HSN tumor-specific antigen
Chondroitin sulfate proteoglycan NG2
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 1L2 is a ZooMab rabbit recombinant monoclonal antibody that specifically detects Chondroitin sulfate proteoglycan 4 (NG2).

免疫原

Recombinant extracellular domain of rat Chondroitin sulfate proteoglycan 4 (NG2).

应用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NG2 in C6 cells.
Immunofluorescence Analysis: A 1:100 dilution representative lot detected NG2 in rat brain tissue sections.
Anti-NG2, clone 1L2 ZooMAb, Cat. No. ZRB5320, is a recombinant Rabbit Monoclonal Antibody that specifically targets Chondroitin sulfate proteoglycan 4 (NG2) and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

目标描述

Chondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Jones, LL et al. (2002). J. Neurosci. 22(7):2792-2803).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1L2, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 251.91 kDa
observed mol wt ~270 kDa
species reactivitymouse, rat
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)immunocytochemistry: suitable using 1:100
immunofluorescence: suitable using 1:100
western blot: suitable using 1:1,000
同位素/亚型IgG
UniProt登记号Q00657
环保替代产品分类Aligned
运输ambient
储存温度2-8℃
Gene Informationrat ... Cspg4;Ng2(81651)

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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