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位置:首页 > 品牌 > Sigma-Aldrich > Anti-Occludin Antibody, clone 1C17 ZooMAb® Rabbit Monoclonal

Anti-Occludin Antibody, clone 1C17 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1451
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
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数量
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产品名称:
Anti-Occludin Antibody, clone 1C17 ZooMAb® Rabbit Monoclonal
OCLN
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 1C17 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Occludin. It targets an epitope within 113 amino acids from the C-terminal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 113 amino acids from the C-terminal region of human Occludin.

应用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Occludin in HEK 293 cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Occludin in human kidney tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound recombinant human Occludin with a KD of 1.1 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Occludin, clone 1C17 ZooMAb, Cat. No. ZRB1451, is a recombinant Rabbit monoclonal antibody that targets Occludin and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Occludin (UniProt: Q16625; also known as OCLN) is encoded by the OCLN gene (Gene ID: 100506658) in human. Occludin is a multi-pass membrane protein that localizes at tight junctions of both epithelial and endothelial cells. It is shown to be highly expressed in kidneys. It plays a role in the formation and regulation of the tight junction paracellular permeability barrier and prevents the transport of macromolecules through the tight junction. Dysregulation of occlude leads to several diseases, including cancer, stroke, and inflammatory lung disease. Occludin has two extracellular domains (aa 90-135 and 196-243), four transmembrane domains (aa 67-89, 136-160, 171-195, and 244-265), and three cytoplasmic domains (aa 1-66, 161-170, and 266-522). The C-terminal cytoplasmic tail binds directly to the ZO-1 proteins, which links the protein complex to the actin cytoskeleton and is involved in the regulation of the permeability barrier function of tight junctions. The first extracellular loop participates in an adhesive interaction. Occludin also exhibits a MARVEL domain (aa 60-269) that is involved in membrane apposition and fusion events. Overexpression of occludin in Madin-Darby canine kidney (MDCK) cells is reported to enhance transepithelial electrical resistance in these cells. Reversible phosphorylation of occluding is shown to play a major role in regulation of occludin and it is tight junction barrier properties. Mutations in OCLN gene can cause Pseudo-TORCH syndrome that is Characterized by microcephaly, intracranial calcifications, and severe developmental delays. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cummins, PM (2012). Mol. Cell Biol. 32(2): 242-250).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1C17, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 59.14 kDa
observed mol wt 55 kDa
species reactivitycanine, human
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1

Sigma-Aldrich

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