产品说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
特异性
Clone 1I4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Synaptic vesicle glycoprotein 2A (SV2A). It targets an epitope within 20 amino acids from the N-terminal, cytoplasmic domain.
免疫原
KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal, cytoplasmic domain of human Synaptic vesicle glycoprotein 2A (SV2A).
应用
Quality Control Testing
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected SV2A (Synaptic Vesicle Glycoprotein 2A) in SH-SY5Y cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SV2A in human brain tissue lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SV2A in E18 cortical and SH-SY5Y cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SV2A in human cerebral cortex and mouse hippocampus tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound SV2A peptides with a KD of 1.4 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-SV2A, clone 1I4 ZooMAb®, Cat. No. ZRB1720, is a recombinant Rabbit monoclonal antibody that specifically targets SV2A and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
目标描述
Synaptic vesicle glycoprotein 2A (UniProt: Q7L0J3; also known as SV2A) is encoded by the SV2A (also known as KIAA0736, PSEC0174) gene (Gene ID: 9900) in human. SV2A is a multi-pass membrane glycoprotein with seven cytoplasmic domains, twelve transmembrane domains, and six extracellular domains. It plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. It is also reported to positively regulate vesicle fusion by maintaining the readily releasable pool of secretory vesicles. SV2A is widely distributed in the nervous system and in endocrine tissue. It is enriched in chromaffin granules and associates with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas. SV2A interact with synaptotagmin-1 in a calcium-dependent manner and this interaction is highly controlled. Phosphorylation of SV2A by casein kinase I in the amino terminal region increases its affinity for synaptotagmin-1. Although its N-terminal has two phosphorylation clusters, only threonine 84 phosphorylation appears to be essential for binding and forms hydrogen bonds with three conserved lysine residues of the C2B domain of synpatotagmin-1. The correct folding of SV2A appears to be important for its function and proper location since many substitution mutations are reported to produce diffuse or aggregated cytoplasmic locations of SV2A. SV2A-knockout mice are reported to develop severe seizures within one postnatal week and death ensues within 2 to 3 weeks. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bartholome, O., et al. (2017). Front. Mol. Neurosci. 10; 148).
外形
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
生物来源 | rabbit |
质量水平 | 200 |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 1I4, monoclonal recombinant monoclonal |
产品线 | ZooMAb® learn more |
形式 | lyophilized |
分子量 | calculated mol wt ~ 82.70 kDa observed mol wt ~ 78 kDa |
species reactivity | mouse, rat, human |
species reactivity (predicted by homology) | monkey, porcine |
包装 | antibody small pack of 25 μL |
增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
同位素/亚型 | IgG |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich