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位置:首页 > 品牌 > Sigma-Aldrich > Anti-TMEM16A Antibody, clone 3J18, ZooMAb® Rabbit Monoclonal

Anti-TMEM16A Antibody, clone 3J18, ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1171
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
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数量
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产品名称:
Anti-TMEM16A Antibody, clone 3J18, ZooMAb® Rabbit Monoclonal
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 3J18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Transmembrane protein 16A (TMEM16A). It targets an epitope within 15 amino acids from the extracellular domain from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids from the extracellular C-terminal region of human Transmembrane protein 16A (TMEM16A).

应用

Anti-TMEM16A, clone 3J18 ZooMAb , Cat. No. ZRB1171, is a highly specific recombinant rabbit monoclonal antibody that detects Transmembrane protein 16A (TMEM16A) and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:100 dilution from a representative lot detected TMEM16A in human pancreas tissue lysate.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected TMEM16A in human salivary gland tissue sections.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

目标描述

Transmembrane protein 16A (UniProt: Q5XXA6; also known as TMEM16A, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Tumor-amplified and overexpressed sequence 2) is encoded by the ANO1 (also known as DOG1, ORAOV2, TAOS2, TMEM16A) gene (Gene ID: 55107) in human. TMEM16A is a homodimeric multi-pass membrane protein that along with TMEMB16B subunit serves as the calcium-activated chloride channels (CaCC). It is broadly expressed with higher levels in liver, skeletal muscle, and gastrointestinal muscles. It plays a role in transepithelial anion transport and smooth muscle contraction and is also required for the normal functioning of the interstitial cells of Cajal (ICCs) that generate electrical pacemaker activity in gastrointestinal smooth muscles. It also acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. ATP and calmodulin are shown to be essential for TMEM16A activation and the channel activity is inhibited by CFTR protein and by chloride channel inhibitors such as niflumic acid and 4, 4 -diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆3J18, recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 114.08 kDa
observed mol wt ~150 kDa
species reactivityhuman
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable (peptide)
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1

Sigma-Aldrich

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