您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Anti-Ubiquilin 4 Antibody, clone 5K6 ZooMAb® Rabbit Monoclonal

Anti-Ubiquilin 4 Antibody, clone 5K6 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1071
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Ubiquilin 4 Antibody, clone 5K6 ZooMAb® Rabbit Monoclonal
Connexin43-interacting protein of 75 kDa
CIP75
Ataxin-1 ubiquitin-like-interacting protein
A1Up
A1U
Ataxin-1 interacting ubiquitin-like protein
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 5K6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Ubiquitin 4. It targets an epitope within 25 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of human Ubiquitin 4.

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ubiquilin 4 in Raji and HepG2 cell nuclear extract.
Affinity Binding Assay: A representative lot of this antibody bound Ubiquilin 4 peptide with a KD of 4.0 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ubiquilin 4 in MCF-7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Ubiquilin 4, clone 5K6 ZooMAb, Cat. No. ZRB1071, is a recombinant Rabbit monoclonal antibody that specifically targets Ubiquilin 4 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.

目标描述

Ubiquilin-4 (UniProt: Q9NRR5; also known as Ataxin-1 interacting ubiquitin-like protein, A1Up, Ataxin-1 ubiquitin-like-interacting protein, A1U, Connexin43-interacting protein of 75 kDa, CIP75) is encoded by the UBQLN4 (also known as C1orf6, CIP75, UBIN) gene (Gene ID: 56893) in human. Ubiquilin-4, a member of the UbL-UBA protein family, serves as an adaptor in the degradation of specific substrates via the proteasomal pathway. It is highly expressed in pancreas, kidney, skeletal muscle, heart, and throughout the brain. Its levels are reported to be upregulated in aggressive tumors. It serves as a regulator of protein degradation and mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins. It acts by binding polyubiquitin chains of target proteins via its UBA domain and by interacting with subunits of the proteasome via its ubiquitin-like domain. Ubiquilin-4 colocalizes with the proteasome, both in nucleus and cytoplasm and is exported from the nucleus following interaction with DESI1/POST. In response to DNA damage and phosphorylation at serine 318 by ATM, it localizes to the nucleus and is recruited to sites of DNA damage where it represses homologous recombination repair. It acts by binding and removing ubiquitinated MRE11 from damaged chromatin, leading to MRE11 degradation by the proteasome. MRE11 degradation then prevents homologous recombination repair and redirects double-strand break repair toward non-homologous end joining. Mutations in UBQLN4 gene are shown to cause Amyotrophic lateral sclerosis (ALS). Defects in UBQLN4 gene also cause intellectual impairment, growth retardation, microcephaly, and facial dysmorphism. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit
质量水平200
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆5K6, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 63.85 kDa
observed mol wt ~64 kDa
species reactivityhuman
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品