产品说明
一般描述
Full length, recombinant, human PDK1 fused to a His•Tag
包装
5 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.
外形
In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON™ X-100 Detergent, pH 7.5.
重悬
Following initial thaw, aliquot and freeze (-70°C).
其他说明
Scott, M.T., et al. 2002. EMBO J.21, 6771.
Biondi, R.M., et al. 2000. EMBO J.19, 979.
Alessi, D.R., et al. 1997. Curr. Biol.7, 776.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
基本信息
酶学委员会(EC)编号 | 2.7.10 |
产品性质
测定 | ≥90% (SDS-PAGE) |
质量水平 | 100 |
形式 | liquid |
specific activity | ≥75 units/mg protein |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
运输 | wet ice |
储存温度 | −70℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
Sigma-Aldrich