产品说明
一般描述
Full-length, recombinant, human PKCδ expressed in S. frugiperda insect cells using a baculovirus expression system. PKC δ is classified as a novel PKC isozyme because it does not exhibit calcium dependence and it is activated in vitro by phorbol esters. Suitable for studies of PKCδ and its regulation. PKCδ is a Ca2+-independent and phospholipid-dependent enzyme.
包装
5 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PKCε Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.5.
外形
In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON™ X-100 Detergent, pH 7.5.
重悬
Following initial thaw, aliquot and freeze (-70°C).
其他说明
Hug, J. and Sarre, T.F. 1993. Biochem. J. 291, 329.
Kazanietz, M.G., et al. 1993. Mol.Pharmacol. 44, 298.
Koide, H., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 1149.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
产品性质
测定 | ≥95% (SDS-PAGE) |
质量水平 | 100 |
形式 | liquid |
specific activity | ≥800 units/mg protein |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
运输 | wet ice |
储存温度 | −70℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich