产品说明
生化/生理作用
Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.
外形
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.
基本信息
| MDL编号 | MFCD00212975 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 生物来源 | human |
| 重组 | expressed in baculovirus infected Sf21 cells |
| 测定 | ≥97% (SDS-PAGE and N-terminal analysis) |
| 形式 | lyophilized powder |
| 效能 | 0.01-0.05 ng/mL ED50 |
| 分子量 | glycoprotein 75 kDa |
| 包装 | pkg of 5 μg |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | endotoxin, tested |
| UniProt登记号 | P29459, P29460 |
| 储存温度 | −20℃ |
| Gene Information | human ... IL12A(3592), IL12B(3593) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
