产品说明
一般描述
Manufactured for Sigma by Boston Biochem., Inc.
应用
Proteasome Fraction II is ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radiolabeled or immunodetectable substrates. Isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the accumulation of ubiquitin protein conjugates. Typical assay concentration for FII is 0.5 - 4 mg/ml.
生化/生理作用
Protein fraction of a cell extract that binds to anion-exchange resin.
Essentially ubiquitin and ATP free. Contains E1, most E2s, UCHs, and the proteasome 20S and 26S isoforms.
外形
Solution in 25 mM HEPES, pH 7.6.
基本信息
MDL编号 | MFCD03458197 |
产品性质
质量水平 | 200 |
形式 | solution |
浓度 | 5 mg/mL |
储存温度 | −70℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich