产品说明
特异性
Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.
This antibody recognizes Giα-2.
免疫原
Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).
应用
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.
Research Category
Signaling
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer
Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes
质量
Routinely evaluated by Western Blot on Huvec lysates.
Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.
目标描述
Labels a single protein band of 39-42 kDa in bovine and rat membranes.
联系
Replaces: MAB3077
外形
Purified
Format: Purified
Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.
储存及稳定性
Maintain at 2-8°C for 1 year from date of receipt.
分析说明
Control
Huvec cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | L5, monoclonal |
species reactivity | mouse (69%), human (62%), guinea pig (43%), bovine (100%), rat (69%) |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
同位素/亚型 | IgG2b |
NCBI登记号 | NM_002070.2 |
UniProt登记号 | P04899 |
运输 | wet ice |
Gene Information | human ... GNAI2(2771) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich