产品说明
一般描述
This monoclonal antibody was generated using an unmodified peptide corresponding to the first 20 amino acids of histone H3 and is specific for an unmodified epitope in that region.
特异性
This antibody recognizes and is specific for histone H3, Mr 17kDa.
This peptide sequence is identical in a wide range of animal and plant species.
免疫原
KLH conjugated peptide corresponding to amino acids 1-20 [ARTKQTARKSTGGKAPRKQLC] of human histone H3. Clone 6.6.2.
Epitope: a.a. 1-20
应用
Research Sub Category
Transcription Factors
Anti-Histone H3 Antibody, clone 6.6.2 is a Mouse Monoclonal Antibody for detection of Histone H3 also known as H3 histone family 3A & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
质量
Routinely evaluated by western blot on acid extracted lysates from HeLa cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Histone H3 in acid extracted lysates from HeLa cells.
目标描述
17 kDa
外形
Protein G Chromatography
Purified mouse monoclonal IgG1κ in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide and 30% glycerol.
Format: Purified
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
分析说明
Control
HeLa cell extract
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 6.6.2, monoclonal |
species reactivity | vertebrates, plant, human |
manufacturer/tradename | Upstate® |
technique(s) | western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NP_003484 |
UniProt登记号 | Q16695 |
运输 | dry ice |
Gene Information | human ... H3F3B(3021) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
Sigma-Aldrich