产品说明
一般描述
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre-mRNA processing. However, its role in the regulation of gene expression is as yet poorly understood. Proteins of the heterogeneous nuclear ribonucleoparticles (hnRNP) family form a structurally diverse group of RNA binding proteins implicated in various functions. Recently, hnRNP proteins have been shown to hinder communication between factors bound to different splice sites. Conversely, several reports have described a positive role for some hnRNP proteins in pre-mRNA splicing. hnRNP-U, also termed scaffold attachment factor A (SAF-A), binds to pre-mRNA and nuclear matrix/scaffold attachment region DNA elements.
特异性
This antibody recognizes hnRNP U.
免疫原
Recombinant protein corresponding to human hnRNP U.
Epitope: Unknown
应用
Immunoprecipitation
Reported by external laboratory using a represenative lot of this antibody
Immunocytochemistry
Reported by external laboratory using a represenative lot of this antibody
ELISA
Reported by external laboratory using a represenative lot of this antibody
Use Anti-hnRNP U Antibody, clone 3G6 (mouse monoclonal antibody) validated in WB, IP, ICC, ELISA to detect hnRNP U also known as heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Apoptosis - Additional
质量
Evaluated by Western Blot in K562 cell lysate.
Western Blot Analysis: 0.01 µg/ml of this antibody detected hnRNP U on 10 µg of K562 cell lysate.
目标描述
Calculated molecular weight is 90 kDa However protein is usually observed at ~120 kDa (Dreyfuss G, Kim VN, Kataoka N. Nat Rev Mol Cell Biol. 2002 Mar;3(3):195-205.)
外形
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
K562 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 3G6, monoclonal |
species reactivity | human |
technique(s) | ELISA: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG2bκ |
NCBI登记号 | NP_004492.2 |
UniProt登记号 | Q00839 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich