产品说明
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
特异性
This antibody recognizes monomethylated Lys9 at and around the N-terminal of Histone H3.
免疫原
KLH conjugated linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3.
Epitope: monomethylated H3 Lys9
应用
Dot Blot Analysis: AbSurance
Peptide Inhibition: Representative lot data. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in western blot analysis of HeLa acid extracts and various other modified histone peptides.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Use Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306 (Mouse Monoclonal Antibody) validated in WB, PIA to detect monomethyl Histone H3 (Lys9) also known as H3K9me1.
质量
Evaluated by Western Blot on recombinant Histone H3 and HeLa acid extract.
Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3 in 500 ng of recombinant Histone H3 and 10 µg of HeLa acid extract.
目标描述
~17 kDa
外形
Format: Purified
Purified mouse monoclonal IgG2a in PBS with 0.05% sodium azide.
Protein G Purified
储存及稳定性
Stable at 2-8°C for 1 year from date of receipt.
分析说明
Control
Recombinant Histone H3 and HeLa acid extract.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | CMA306, monoclonal |
species reactivity | human |
technique(s) | inhibition assay: suitable (peptide) western blot: suitable |
同位素/亚型 | IgG2a |
NCBI登记号 | NP_003484 |
UniProt登记号 | O43463 |
运输 | wet ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich