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Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat

品牌
Sigma-Aldrich
货号
F2653
规格纯度
flow cytometry grade, affinity isolated antibody, buffered aqueous solution
参考价格
4729.39 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat
抗-小鼠IgG (Fab特异性) F(ab′)2片段–FITC 山羊抗
产品介绍:

产品说明

一般描述

IgG, a monomer, is the principal antibody circulating in blood which assist in phagocytic destruction of foreign microorganism and is the only class of Ig that can cross the placenta in humans. Cell-sorting done by differential binding of anti-mouse IgG (Fab Specific) - FITC antibody separates tumour cells from macrophages. This antibody can be used to incubate somatic cell nuclear transfer (SCNT) rat embryos for immunocytochemical observation. It also can be used as a secondary antibody to study the dynamics of activated B lymphocytes in small intestine, Peyer′s patch germinal center as well as non-germinal center, spleen and the mesenteric lymph node. Anti-mouse IgG antibody reacts specifically with all mouse immunoglobulins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

特异性

Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.

免疫原

Purified Fab fragments of mouse IgG.

应用

Anti-Mouse IgG (Fab Specific) FITC Conjugate antibody is used in traditional method of immunocytochemical assessment of ZAP-70 expression. It can also be used in immunocytochemical observation of the SCNT embryos.
Anti-Mouse IgG (Fab specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in:

  • indirect immunofluorescence staining
  • immunofluorescence staining
  • flow cytometry


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

生化/生理作用

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

其他说明

Antibody adsorbed with bovine, equine and human serum proteins.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

制备说明

Adsorbed to reduce background staining with bovine, horse or human samples.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162622
NACRESNA.46

产品性质

质量水平200
生物来源goat
偶联物FITC conjugate
抗体形式affinity isolated antibody
antibody product typesecondary antibodies
等级flow cytometry grade
克隆polyclonal
形式buffered aqueous solution
technique(s)flow cytometry: 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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