产品说明
一般描述
Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.
特异性
Recognizes phosphorylated serines or threonines when followed by a proline.
免疫原
Mitotic human HeLa cell cytosolic lysate
应用
Anti-phospho-Ser/Thr-Pro MPM-2 Antibody is a Mouse Monoclonal Antibody for detection of phospho-Ser/Thr-Pro also known as Mitotic protein #2 & has been tested in ELISA, IHC, IP & WB.
Research Sub Category
Cell Cycle, DNA Replication & Repair
Research Category
Epigenetics & Nuclear Function
质量
routinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells
目标描述
varies depending on the protein you are detecting
外形
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Format: Purified
Protein G Purified
储存及稳定性
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
克隆 | MPM-2, monoclonal |
species reactivity | vertebrates |
manufacturer/tradename | Upstate® |
technique(s) | ELISA: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1 |
运输 | wet ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
Sigma-Aldrich