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位置:首页 > 品牌 > Sigma-Aldrich > Anti-SMAD1 Antibody, clone AS22

Anti-SMAD1 Antibody, clone AS22

品牌
Sigma-Aldrich
货号
05-1459
规格纯度
clone AS22, from mouse
参考价格
5520.38 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-SMAD1 Antibody, clone AS22
SMAD 1, SMAD family member 1, SMAD, mothers against DPP homolog 1, MAD, mothers against decapentaplegic homolog 1, Mad-related protein 1, Mothers against DPP homolog 1, Sma- and Mad-related protein 1, TGF-beta signaling protein 1, Transforming growth fac
产品介绍:

产品说明

一般描述

Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.

特异性

Detects SMAD1
Other species were not tested.

免疫原

GST-fusion protein corresponding to amino acids 1-110 of human SMAD1.

应用

Western Blot Analysis:
This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.
Research Category
Signaling
Anti-SMAD1 Antibody, clone AS22 detects level of SMAD1 & has been published & validated for use in WB & IF.

质量

Routinely evaluated by Western Blot
Western Blot Analysis:
This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.

目标描述

52 kDa

联系

Replaces: 04-1100

外形

Purified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆AS22, monoclonal
species reactivitymouse, human
technique(s)immunofluorescence: suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_001003688
UniProt登记号Q15797
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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