产品说明
一般描述
The TATA-box-binding protein (TBP) is a critical, eukaryotic transcription factor. TBP a part of the RNA polymerase II eukaryotic transcription complex and is required for the transcriptional initiation of RNA polymerases. TBP is considered to be a limiting factor for certain RNA promoters. Expression is ubiquitous, however the highest expression has been observed in testis and ovary. Mutations in TBP result in spinocerebellar ataxia type 17(SCA17), a cerebellar disorder classified as autosomal dominant cerebellar ataxia and characterized by atrophy to the cerebellar and cerebral regions, extrapyramidal signs, and dementia.
特异性
This antibody recognizes TATA-box-binding protein at the N-terminus.
免疫原
GST-tagged recombinant protein corresponding to human TATA-box-binding protein at the N-terminus.
Epitope: N-terminus
应用
Research Sub Category
Transcription Factors
Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in U2OS cells. (Denisso, S., et al. (2007). The EMBO Journal. 26:944–954.)
Research Category
Epigenetics & Nuclear Function
Use Anti-TATA-box-binding protein Antibody, clone SL30-3-563 (mouse monoclonal antibody) validated in WB to detect TATA-box-binding protein also known as TATA box binding protein, Transcription initiation factor TFIID TBP subunit.
质量
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected TATA-box-binding protein on 10 µg of HeLa cell lysate.
目标描述
~38 kDa observed.
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HeLa cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | SL30-3-563, monoclonal |
species reactivity | human |
species reactivity (predicted by homology) | mouse (based on 100% sequence homology), rat (based on 100% sequence homology) |
technique(s) | ChIP: suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NP_003185 |
UniProt登记号 | P20226 |
运输 | wet ice |
Gene Information | human ... TBP(6908) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich