产品说明

一般描述

Nanodiscs are non-covalent structures that contain a phospholipid bilayer and a membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence.

应用

纳米盘可溶性脂质双层系统已被证明是一种广泛适用的方法，用于呈现膜蛋白可溶于水溶液中，在类似原生的双层环境中保持单分散和活性。纳米盘的关键组成部分是环绕的两亲性螺旋蛋白带(膜支架蛋白)。
纳米盘系统已经被用于包括 GPCRs 、P450 、细菌视紫红质 、凝血因子 、霍乱毒素 、焦油受体 和芳香化酶 等多种蛋白质的掺入。
For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

生化/生理作用

生成直径约为 9.7 nm 的纳米盘

法律信息

纳米盘技术，以及它的许多用途，都被伊利诺伊大学拥有的以下专利所涵盖。

• 7,691,414 膜支架蛋白
• 7,662,410 膜支架蛋白和包埋膜蛋白
• 7,622,437 组织因子组成和方法
• 7,592,008 膜支架蛋白
• 7,575,763 膜支架蛋白和栓系膜蛋白
• 7,083,958 膜支架蛋白
• 7,048,949 膜支架蛋白

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.

• 7,691,414 Membrane scaffold proteins
• 7,662,410 Membrane scaffold proteins and embedded membrane proteins
• 7,622,437 Tissue factor compositions and methods
• 7,592,008 Membrane scaffold proteins
• 7,575,763 Membrane scaffold proteins and tethered membrane proteins
• 7,083,958 Membrane scaffold proteins
• 7,048,949 Membrane scaffold proteins

产品性质

质量水平	200
重组	expressed in E. coli
测定	≥90% (SDS-GE)
形式	buffered aqueous solution
运输	ambient
储存温度	−20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable