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Membrane Scaffold Protein 1E3D1 D73C

品牌
Sigma-Aldrich
货号
MSP09
规格纯度
recombinant, expressed in E. coli, Cysteine substituted at position 73
参考价格
8122.7 *本价格含增值税费
促销
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数量
-+
产品名称:
Membrane Scaffold Protein 1E3D1 D73C
产品介绍:

产品说明

一般描述

Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1).
The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E3D1 D73C variant of MSP1 differs from MSP1 in the following facets:

  • It deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence3 (which is known separately as MSP1D1).
  • It repeats the Helix 4 (H4), Helix 5 (H5) and Helix 6 (H6) sequences of the original MSP1 sequence between the parent Helix 6 (H6) and Helix 7 (H7) segments of MSP1D1.
  • It substitutes a cysteine (C) residue for an aspartic acid (D) residue in the Helix 2 (H2) portion of the protein, at position 73 of the original native A-1 sequence.
  • The initial histidine-tag is one amino acid shorter.

应用

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

法律信息

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.

  • 7,691,414 Membrane scaffold proteins
  • 7,662,410 Membrane scaffold proteins and embedded membrane proteins
  • 7,622,437 Tissue factor compositions and methods
  • 7,592,008 Membrane scaffold proteins
  • 7,575,763 Membrane scaffold proteins and tethered membrane proteins
  • 7,083,958 Membrane scaffold proteins
  • 7,048,949 Membrane scaffold proteins

基本信息

NACRESNA.26

产品性质

质量水平200
重组expressed in E. coli
测定≥90% (SDS-GE)
形式buffered aqueous solution
运输ambient
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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