产品说明
一般描述
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains an n-terminal Hexa-Histidine (6His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHH.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
序列
To view sequence information for this product, please visit the product page
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
基本信息
| NACRES | NA.85 |
产品性质
| 标签 | 6-His tagged |
| 形式 | buffered aqueous solution |
| 分子量 | size 6167 bp |
| 复制起点 | pUC (500 copies) |
| 肽切割 | no cleavage |
| 肽标签位置 | N-terminal |
| 启动子 | Promoter name: CMV Promoter activity: constitutive Promoter type: mammalian |
| 菌种筛选 | kanamycin |
| 哺乳动物细胞筛选 | puromycin |
| 报告基因 | none |
| 运输 | ambient |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
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