产品说明
生化/生理作用
Human DNA topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. The core domain spanning from amino acids 215 to 636 is highly conserved and retains DNA binding activity. Topo I has been found to nick the DNA with a preference of 5′-(A/T)(G/C)(A/T)T-3′. Camptothecin and its analogs have been tested as potent anticancer compounds by stabilizing topo I-DNA complex, thereby inhibiting both DNA and RNA synthesis.
外形
Clear and colorless frozen liquid solution
制备说明
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
产品性质
| 生物来源 | human |
| 重组 | expressed in insect cells |
| 测定 | ≥80% (SDS-PAGE) |
| 形式 | frozen liquid |
| 分子量 | ~56 kDa |
| 包装 | pkg of 5 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 浓度 | 500 μg/mL |
| 颜色 | clear colorless |
| NCBI登记号 | NM_003286 |
| UniProt登记号 | P11387 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... TOP1(7150) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
