Anti-MDC1 Antibody, clone P2B11

产品说明

一般描述

MDC1 is a subunit of the cohesin complex and is an essential protein required for sister chromatid cohesion in mitosis and meiosis. Temperature-sensitive mutations of this protein have been associated with defects in sister chromatid cohesion, chromosome condensation, and chromosome segregation. Its expression is cell cycle regulated and peaks in S phase. Expression of MDC1 is required for the S- and G2/M-checkpoint-mediated cell cycle arrest in response to DNA damage. MDC1 has been shown to interact with H2A.X near sites of DNA double-strand breaks and recruit ATM kinase to the damaged region of DNA. Consistent with this role, MDC1 also appears to modulate the function of other proteins involved in the DNA damage checkpoint such as BRCA1 and Chk2.

特异性

This antibody recognizes MDC1 at and around the N-terminus.

免疫原

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.

应用

Anti-MDC1 Antibody, clone P2B11 is a Mouse Monoclonal Antibody for detection of MDC1 also known as Nuclear factor with BRCT domains 1 & has been validated in ICC & WB.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected MDC1 in NIH/3T3 cells.
Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Lou, Z., et al. (2003). Nature. 421(6926):957-961.)

目标描述

~ 184 kDa There are 4 isoforms of human MDC1 because of splicing with molecular weights of approximately 227, 198, 196, and 117 kDa

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) without 0.05% sodium azide.
Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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