Anti-Ras Antibody, clone RAS10

产品说明

一般描述

The tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals.

特异性

Other species unknown.

免疫原

Purified recombinant Ras. Clone RAS10.

应用

Detect Ras using this Anti-Ras Antibody, clone RAS10 validated for use in ELISA, FC, IC, IH, IP & WB.
Immunoprecipitation:
1-4 μg of this antibody has been reported to immunoprecipitate Ras.
Immunocytochemistry:
2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells.
Immunohistochemistry:
This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.

质量

Routinely evaluated by western blot on RIPA lysate from human A431 carcinoma cells, human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 or PC-12 cell lysates.
Western Blot Analysis:
0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.

目标描述

21 kDa

联系

Replaces: CBL751

外形

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2ak in buffer containing PBS, pH 7.4, with 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

分析说明

Control
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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