产品说明
一般描述
Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.
应用
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.
亲和素蛋白用于一项分析中，该分析使用官能化氙作用检测生物素-亲和素结合的生物传感器。使用80nmol的鸡蛋白。
包装
5 mg in glass bottle
单位定义
One unit will bind 1.0 μg of d-biotin.
制备说明
亲和纯化
基本信息
| EC 号 | 215-783-6 |
| MDL编号 | MFCD00130572 |
| NACRES | NA.56 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in corn |
| 测定 | ≥80% protein basis (biuret) |
| 形式 | powder |
| specific activity | ≥12 units/mg protein (E |
| 分子量 | glycoprotein 66 kDa subunit 16 kDa |
| technique(s) | ELISA: suitable immunoblotting: suitable immunoelectrophoresis: suitable |
| UniProt登记号 | P02701 |
| 储存温度 | 2-8℃ |
| Gene Information | chicken ... AVD(396260) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
