产品说明
一般描述
Product has lost contact inhibition
细胞系来源
Mouse Swiss Albino embryo fibroblast
细胞系描述
Derived from Swiss mouse embryos this cell line secretes collagen and hyaluronic acid. Unlike 3T3, the 3T6 line is not contact inhibited.
培养基
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
产品性质
| 生物来源 | mouse embryo |
| 生长模式 | Adherent |
| 核型 | 2n = 40 |
| 形态学 | Fibroblast |
| 产品 | Collagen and hyaluronic acid |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | −196℃ |
Sigma-Aldrich
m.cnreagent.com
