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Anti-EF1α Antibody, clone CBP-KK1

品牌
Sigma-Aldrich
货号
05-235
规格纯度
clone CBP-KK1, Upstate®, from mouse
参考价格
5206.52 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-EF1α Antibody, clone CBP-KK1
CTCL tumor antigen, EF1a-like protein, Elongation factor 1 A-1, Elongation factor Tu, Leukocyte receptor cluster member 7, cervical cancer suppressor 3, elongation factor 1 alpha subunit, elongation factor 1-alpha, eukaryotic translation elongation facto
产品介绍:

产品说明

一般描述

Human eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The alpha 1 isoform is expressed in brain, placenta, lung, liver, kidney, and pancreas. The other isoform (alpha 2) is expressed in brain, heart and skeletal muscle.
The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer.

特异性

This antibody recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa. Occasionally, a 38 kDa protein is also detected in A431 cell lysate.

免疫原

Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography. Clone CBP-KK1.

应用

Immunoprecipitation:
4 µg of a previous lot immunoprecipitated EF1α from an A431 RIPA cell lysate.
Research Category
Protein Trafficking
Research Sub Category
RNA Metabolism & Binding Proteins

RNA Binding Protein (RBP)
Detect EF1α using this Anti-EF1α Antibody, clone CBP-KK1 validated for use in IP & WB.

质量

Routinely evaluated on RIPA lysates from non-stimulated A431 cells, human Jurkat or mouse 3T3/A31.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected EF1α in RIPA lysates from non-stimulated A431 cells. Previous lots detected EF1α in human Jurkat and mouse 3T3/A31 RIPA cell lysates.

目标描述

53 kDa

外形

Protein G Chromatography
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide.
Format: Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Jurkat cell lysate.
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol per 100 µL of lysate and boil for 5 minutes to reduce the preparation. Use 20 µg per lane for minigels.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
600
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆CBP-KK1, monoclonal
species reactivitySaccharomyces cerevisiae, bacteria
species reactivity (predicted by homology)mammals
manufacturer/tradenameUpstate®
technique(s)immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NM_006620
UniProt登记号P68104
运输dry ice
Gene Informationhuman ... EEF1A1(1915)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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