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C99

货号
CB_12022905
规格纯度
12022905, from human colon
参考价格
4474.8 *本价格含增值税费
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数量
-+
产品名称:
C99
C-99
C 99
产品介绍:

产品说明

细胞系来源

Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage C

细胞系描述

The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes′ stage C.

DNA图谱分析

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 8,14
D16S539: 11,13
D5S818: 11,12
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,10
vWA: 16,17

培养基

Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 ℃. C99 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

产品性质

生物来源human colon
生长模式Adherent
核型Not specified
形态学Epithelial
产品Not specified
受体Not specified
technique(s)cell culture | mammalian: suitable
相关疾病cancer
运输dry ice

Sigma-Aldrich

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