产品说明
一般描述
Homozygous: No
Consanguineous: No
细胞系描述
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA图谱分析
A: 201
Cw: 702
B: 702
HLA DQA1: 3011
HLA DPB1: 201
血清学分析
HLA A: 2
HLA C: 7
HLA B: 7
Bw4/bw6: 6
HLA DR: 4 13
HLA DR52: 52
HLA DR53: 53
HLA DQ: 1 3
培养基
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
传代培养常规
When resuscitating from frozen, cells should be seeded at 0.5 x 106 cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 105 cells/ml. Maintain cultures between 3-9 x 105 cells/ml for maximum growth; 5% CO2; 37℃. Cells form floating aggregates of varying size.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免责声明
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
产品性质
生物来源 | human blood |
种族 | Amish |
性别 | male |
生长模式 | Suspension |
核型 | Not specified |
形态学 | Lymphoblastoid |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
Sigma-Aldrich