产品说明
细胞系来源
Human Caucasian ovary teratocarcinoma
细胞系描述
Derived from ascitic fluid cells of a 12 year old Caucasian female with teratocarcinoma of the ovary. The cells produce tumours in nude mice.
应用
Tumourigenicity studies
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 9,10
D16S539: 9,12
D5S818: 11
D7S820: 9
THO1: 7,9
TPOX: 11
vWA: 15,17
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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产品性质
生物来源 | human ovary |
生长模式 | Adherent |
核型 | 2n = 46, pseudodiploid |
形态学 | Epithelial |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | −196℃ |
Sigma-Aldrich