产品说明
包装
25 units in serum bottle
应用
适用于通过 Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974) 中介绍方法的测定乙酰乙酸酯和
生化/生理作用
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
单位定义
One unit will oxidize 1.0 μmole of
外形
含 Tris 缓冲盐的冻干粉
基本信息
酶学委员会(EC)编号 | 1.1.1.30 ( BRENDA | IUBMB ) |
EC 号 | 232-830-6 |
MDL编号 | MFCD00130633 |
NACRES | NA.54 |
产品性质
质量水平 | 200 |
类型 | Type V |
形式 | lyophilized powder |
specific activity | 250-750 units/mg protein |
纯化方式 | chromatography |
异质活性 | lactate dehydrogenase ≤0.05% malate dehydrogenase ≤0.1% |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich