产品说明
一般描述
Sulforhodamine B (also known as SRB, kiton rhodamine B, lissamine rhodamine B, or sulphorhodamine B) is a bright-pink anionic, aminoxanthene dye carrying two sulfonate substituents.
应用
Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.
It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.
It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.
包装
5, 25 g in glass bottle
基本信息
经验(实验)分子式 | C27H29N2NaO7S2 |
分子量 | 580.65 |
颜色索引号 | 45100 |
Beilstein | 3886008 |
EC 号 | 222-529-8 |
MDL编号 | MFCD00010180 |
PubChem化学物质编号 | 24899824 |
NACRES | NA.47 |
产品性质
质量水平 | 200 |
形式 | powder |
组成 | Dye content, ~75% |
εmax | ≥85000 at 554-560 nm |
application(s) | diagnostic assay manufacturing hematology histology |
储存温度 | room temp |
SMILES string | [Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1 |
InChI | 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1 |
InChI key | SXQCTESRRZBPHJ-UHFFFAOYSA-M |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich