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Pronase® Protease, Streptomyces griseus, Nuclease-Free

品牌
Millipore
CAS
9036-06-0
货号
537088
规格纯度
Widely used broad-spectrum protease mixture
参考价格
1152.58 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
9036-06-0
Pronase® Protease, Streptomyces griseus, Nuclease-Free
产品介绍:

产品说明

一般描述

PRONASE® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.

应用

Pronase® Protease, Streptomyces griseus, nuclease-free has been used:

  • in single-cell preparations for cardiac side population (CSP) analysis
  • in aflatoxin plasma biomarker assay
  • to permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion

包装

10, 100, 5, 50 ku in Plastic ampoule
25 ku in Glass bottle

生化/生理作用

Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.

警告

Toxicity: Harmful (C)

单位定义

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

重悬

Unstable in solution; reconstitute just prior to use.

其他说明

Note: 1 KU = 1000 units.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

MDL编号MFCD00132092

产品性质

形式lyophilized
specific activity≥70,000 proteolytic units/g dry wt
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
溶解性water: 10 mg/mL
异质活性Nuclease, none detected
运输ambient
储存温度2-8℃

安全信息

象形图GHS07GHS08
警示用语:Danger
危险声明H315 - H319 - H334 - H335
预防措施声明P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
危险分类Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官Respiratory system
储存分类代码11 - Combustible Solids
WGKWGK 2

Sigma-Aldrich

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