产品说明
应用
α-Chymotrypsin agarose from bovine pancreas has been used to study the purification and characterization of glucoamylase. α-Chymotrypsin agarose from bovine pancreas has also been used in a study to investigate molecular modeling for the design of a biomimetic chimeric ligand.
包装
50, 100 units in poly bottle
生化/生理作用
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
Serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the bond.
单位定义
One unit will hydrolyze 1.0 μmole of N-acetyl-L-tyrosine ethyl ester (ATEE) per min at pH 8.0 at 30 ℃.
外形
Stabilized with lactose
分析说明
由 A
基本信息
| 酶学委员会(EC)编号 | 3.4.21.1 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00130481 |
产品性质
| 形式 | lyophilized powder |
| 质量水平 | 200 |
| specific activity | 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units) |
| 分子量 | 25 kDa |
| UniProt登记号 | P00767 |
| 储存温度 | −20℃ |
| Gene Information | cow ... CTRB1(618826) |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H315 - H317 - H319 - H334 - H335 |
| 预防措施声明 | P280 - P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 个人防护装备 | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
Sigma-Aldrich
m.cnreagent.com
