产品说明
应用
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P4406 is agarose-linked papain and is provided as a lyophilized powder stabilized with lactose. Product P4406 has been used to digest purified anti-JMJD6 hamster monoclonal antibody into Fab fragments.
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
包装
25, 50, 100 units in glass bottle
生化/生理作用
Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
单位定义
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 6.2 at 25℃, unless otherwise indicated below.
外形
Lyophilized powder, stabilized with lactose
基本信息
| MDL编号 | MFCD00131791 |
| NACRES | NA.54 |
产品性质
| 形式 | lyophilized powder |
| 质量水平 | 200 |
| specific activity | 90-150 units/mL packed gel |
| 分子量 | 21 kDa |
| 标记范围 | 3,000-5,000 units per g agarose (at pH7.0 at 30 ℃.) |
| 基质 | beaded agarose |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H317 - H334 |
| 预防措施声明 | P261 - P280 - P342 + P311 |
| 危险分类 | Resp. Sens. 1 - Skin Sens. 1 |
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
Sigma-Aldrich
m.cnreagent.com
