产品说明
一般描述
Anti-Rabex-5 (N-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to a sequence at N-terminal of human Rabex-5, with an added cysteine, conjugated to KLH. Rabex-5 contains multiple functional domains, including a vacuolar protein sorting-associated (VPS9) domain that possesses the GDP/GTP exchange factors (GEF) activity, a ubiquitin-binding A20-like zinc finger (ZnF) domain, and a coiled-coil domain.
应用
Anti-Rabex-5 (N-terminal) antibody may be used in immunoblotting and immunofluorescence.
生化/生理作用
Rabex-5 forms a tight complex with Rabaptin-5 that is essential for endocytic membrane fusion. The bound Rabaptin-5 to Rabex-5 increases the GEF activity of Rabex-5 and fastens the generation of the active GTP-bound form of membrane associated Rab5.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~58 kDa |
| species reactivity | rat, human, mouse |
| 浓度 | ~1.0 mg/mL |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin western blot: 2-5 μg/mL using whole extracts of mouse brain and human U87 cells |
| UniProt登记号 | Q9UJ41 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... RABGEF1(27342) mouse ... Rabgef1(56715) rat ... Rabgef1(360797) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
