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Anti-Thymine-DNA Glycosylase Antibody

品牌
Sigma-Aldrich
货号
ABE1440
规格纯度
from rabbit, purified by affinity chromatography
参考价格
5520.38 *本价格含增值税费
促销
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数量
-+
产品名称:
Anti-Thymine-DNA Glycosylase Antibody
G/T mismatch-specific thymine DNA glycosylase, hTDG, Thymine-DNA glycosylase
产品介绍:

产品说明

一般描述

G/T mismatch-specific thymine DNA glycosylase (EC 3.2.2.29; UniProt Q13569; also known as hTDG, Thymine-DNA glycosylase) is encoded by the TDG gene (Gene ID 6996) in human. Base excision repair (BER) is initiated by damage-specific DNA glycosylases that recognize and remove aberrant bases by hydrolyzing the N-glycosidic bond, creating an apurinic/apyrimidinic (AP) site. This is followed by an (AP)-endonuclease-catalyzed DNA strand incision 5′ to the AP site, trimming of the 5′ end by an (AP)-lyase or an endonuclease, DNA resynthesis by a DNA polymerase and strand sealing by a DNA ligase. Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases possess additional AP lyase activity and can therefore convert a base lesion into a single-strand break without the need for a separate AP endonuclease. TDG is a monofunctional DNA glycosylase that excises thymine and uracil from G·T and G·U mismatched oligonucleotide substrates as well as the highly mutagenic adduct 3,N4-ethenocytosine from double-stranded DNA. TDG DNA-binding affinity is regulated by SUMOylation, which dramatically reduces its affinity toward DNA substrate and AP site. Sumoylation also potentiates the stimulatory effect of (AP)-endonuclease (APE1/HAP1) on TDG.

免疫原

KLH-conjugated linear peptide corresponding to a sequence from the N-terminal region of human Thymine-DNA Glycosylase.
Epitope: N-terminal

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Immunocytochemistry Analysis: 2.5 µg/mL from a representative lot detected Thymine-DNA Glycosylase in A431, HeLa, HUVEC, and NIH/3T3 cells.
Anti-Thymine-DNA Glycosylase Antibody is an antibody against Thymine-DNA Glycosylase for use in Western Blotting, Immunocytochemistry.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Thymine-DNA Glycosylase in 10 µg of HeLa nuclear extract.

目标描述

~55 kDa observed. 46.05 kDa calculated. 55–60 kDa unmodified and 81-86 kDa posttranslationally modified forms reported (Hardeland, U., et al. (2002). EMBO J. 21(6): 1456-1464). Uncharacterized band(s) may appear in some lysates.

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, human
technique(s)immunocytochemistry: suitable
western blot: suitable
NCBI登记号NP_003202
UniProt登记号Q13569
运输wet ice
Gene Informationhuman ... TDG(6996)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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