Anti-MeCP2 antibody, Mouse monoclonal antibody produced in mouse

产品说明

一般描述

Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Mec-168 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C- terminus of human MeCP2. Methyl-CpG binding protein 2 (MeCP2) is the first methyl-CpG-binding protein to be isolated. This protein contains a methyl-CpG-binding domain (MBD) and a transcriptional repression domain (TRD).

免疫原

synthetic peptide corresponding to the C-terminus (amino acids 471-486) of human MeCP2.

应用

Monoclonal Anti-MeCP2 antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• immunostaining
• enzyme linked immunosorbent assay (ELISA)
• immunocytochemistry
• western blot (0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)

生化/生理作用

Methyl-CpG binding protein 2 (MeCP2) is a transcription modulator that binds methylated DNA. This protein regulates neuronal functions and central nervous system development. Alterations in MeCP2 have been associated with neurological diseases such as MECP2 duplication syndrome and Rett syndrome
Methyl-CpG binding protein 2 (MeCP2) deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes. Antibodies reacting specifically with MeCP2 may be used for studying chromatin remodeling effects on gene expression.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD02686372
NACRES	NA.41

产品性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified from hybridoma cell culture
antibody product type	primary antibodies
克隆	Mec-168, monoclonal
形式	buffered aqueous solution
分子量	antigen ~75 kDa
species reactivity	rat, mouse, human
包装	antibody small pack of 25 μL
technique(s)	immunocytochemistry: suitable indirect ELISA: suitable microarray: suitable western blot: 0.1-0.2 μg/mL using nuclear cell extract of cultured Jurkat, human acute T cell leukemia cells, or cultured MCF7 human breast carcinoma.
同位素/亚型	IgG1
UniProt登记号	P51608
运输	dry ice
储存温度	−20℃
Gene Information	human ... MECP2(4204) mouse ... Mecp2(17257) rat ... Mecp2(29386)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)