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Anti-DNA polymerase δ p50 antibody, Rat monoclonal

品牌
Sigma-Aldrich
货号
SAB4200054
规格纯度
clone PDK 7B4, purified from hybridoma cell culture
参考价格
5045.46 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-DNA polymerase δ p50 antibody, Rat monoclonal
Anti-POLD2
Monoclonal Anti-DNA polymerase δ p50 antibody produced in rat
Anti-Polymerase (DNA directed), delta 2, regulatory subunit 50kDa
产品介绍:

产品说明

一般描述

Monoclonal Anti-DNA polymerase δ p50 (rat IgG2a isotype) is derived from the hybridoma PDK 7B4 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p50. DNA polymerase δ consists of the proliferating cell nuclear antigen (PCNA), the multi-subunit replication factor C, and the 4 subunit DNA polymerase complex: DNA Polymerase δ 1, 2, 3, 4 (POLD1, POLD2, POLD3 and POLD4). The core DNA polymerase δ exists as a heterodimeric enzyme with subunits of 125 and 50 kDa.
The DNA polymerase δ 2 subunit (POLD2) gene, with 11 exons spanning 10kb of genomic DNA, is mapped to human chromosome 7. The gene encoding 50kDa protein is characterized with the G+C-rich 5′-flanking region and it lacks a typical TATA box. The encoded protein is one of the components of human DNA Pol δ complex.

免疫原

human p50

应用

Monoclonal Anti-DNA polymerase δ p50 antibody has been used in

  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • western blot analysis

生化/生理作用

DNA polymerase δ 2 subunit (POLD2) gene codes for a protein that participates in DNA replication and repair. In COS-7 cells, regulatory region of the hPOLD2 gene has ability to facilitate the expression of chloramphenicol acetyltransferase reporter gene. Phosphatase and tensin homolog (PTEN) tumor suppressor gene decrease the expression of POLD2 gene associated with ovarian carcinogenesis. This protein might also function as a potential prognostic biomarker in ovarian carcinoma. POLD2 takes part in polymeraseδ (Pol δ) assembly by coordinating simultaneously with all other three subunits of the enzyme.

外形

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.41

产品性质

质量水平200
生物来源rat
偶联物unconjugated
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆PDK 7B4, monoclonal
形式buffered aqueous solution
分子量antigen ~50 kDa
species reactivityhuman
包装antibody small pack of 25 μL
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using HeLa cell extracts
同位素/亚型IgG2a
UniProt登记号P49005
运输dry ice
储存温度−20℃
Gene Informationhuman ... POLD2(5425)

安全信息

储存分类代码12 - Non Combustible Liquids
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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