产品说明
一般描述
Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the hybridoma MBP-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant MBP fusion protein. Fibronectin (FN), also known as Cold-insoluble globulin (CIG), is a multi-domain glycoprotein composed of two nearly identical disulfide-bound polypeptides. It is widely expressed by multiple cell types and is critically important in vertebrate development, as demonstrated by the early embryonic lethality of mice with targeted inactivation of the FN1 gene 6. In vertebrates, two types of Fibronectin are present: soluble plasma Fibronectin and insoluble cellular Fibronectin. The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue.
特异性
Anti-Fibronectin antibody, Mouse monoclonal specifically recognizes Fibronectin from plasma and in the pericellular extracellular matrix of cultured fibroblasts from human and mouse origin.
免疫原
Fibronectin isolated from human plasma.
应用
The antibody may be used in various immunochemical techniques including Immunoblotting (∼220-260kDa)1, ELISA, Immunofluorescence, Immunohistochemistry and Immunoprecipitation.
生化/生理作用
The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue.
外形
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
其他说明
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | FN-15, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | 220-260 kDa |
| species reactivity | mouse, human |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoblotting: 0.3-0.6 μg/mL using human fetal fibroblast-like WI-38 cell line extract immunofluorescence: 2.5-5 μg/mL using human foreskin fibroblast Hs68 cells immunohistochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: 0.15-0.3 μg/mL using 5 μg/mL Fibronectin from human plasma for coating |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P02751 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... FN1(2335) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
