产品说明
一般描述
NUCB2 (N-terminal) (nucleobindin 2, also known as NEFA, p54) belongs to the nucleobindin family of EF-hand Ca2+-binding proteins.
Nucleobindin 2 (NUCB2), contain multiple functional domains, including a Leu/Ile-rich region, a putative nuclear localization signal, a DNA binding domain, two Ca2+ -binding EF-hand motifs, and a leucine zipper region. NUCB2 is widely distributed and is localized to the Golgi apparatus. Human NUCB2 has high homology (62% sequence identity) with human NUCB1, an additional member of the nucleobindin family, although they are encoded by two separate genes.
应用
Anti-NUCB2 (N-terminal) antibody produced in rabbit has been used in:
生化/生理作用
Nucleobindin 2 (NUCB2) is implicated in various functions, including Ca2+ homeostasis, hypothalamic regulation of feeding, and tumor necrosis factor (TNF)-receptor-1 (TNFR1) shedding. NUCB2 has been shown to interact with both necdin and endoplasmic reticulum aminopeptidase 1 (ARTS-1), both through the EF-hand motif. NUCB2-ARTS-1 complex is required for the proteolytic cleavage and release of TNFR1 to the extracellular compartment. NUCB2 has been recently identified as a novel satiety molecule in the hypothalamus, associated with melanocortin signaling in the central nervous system. NUCB2 and its N-terminal 82-amino acid cleavage fragment, termed nesfatin-1, have been shown to induce suppression of food-intake in rats.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
NACRES | NA.44 |
产品性质
生物来源 | rabbit |
质量水平 | 200 |
偶联物 | unconjugated |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | buffered aqueous solution |
分子量 | antigen ~54 kDa |
species reactivity | mouse, rat |
浓度 | ~1.5 mg/mL |
technique(s) | indirect immunofluorescence: 1-2 μg/mL using Rat2 cells western blot: 1.5-3.0 μg/mL using Rat2 cell lysates western blot: 2-4 μg/mL using mouse testis extracts (S1 fraction) |
UniProt登记号 | P81117 |
运输 | dry ice |
储存温度 | −20℃ |
Gene Information | rat ... Nucb2(59295) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich