产品说明
一般描述
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble form is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
特异性
Reacts with mouse SCF; SCF is highly conserved cross reactivity with rat, human and other species SCF is expected but untested.
免疫原
Highly pure (>98%) recombinant mouse SCF
应用
Research Sub Category
Hematopoietic Stem Cells
Detect Stem Cell Factor using this Anti-Stem Cell Factor Antibody validated for use in ELISA, WB, NEUT.
Research Category
Stem Cell Research
ELISA: To detect mSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of at lease 0.5 μg/mL of this antibody is required. Should allow detection of 0.2 - 0.4 ng/well of recombinant mSCF.
Neutralization: For ½ maximal inhibition (ND50) of the bioogical activity of mSCF (20 ng/mL), a concentration of 0.64 - 0.97 μg/mL is required.
Western blot: 0.1 - 0.2 μg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Optimal working dilutions must be determined by the end user.
目标描述
30 kDa
外形
Affinity purified immunoglobulin. Lyophilized from 0.5X PBS pH 7.2. Reconstitute with sterile distilled water.
储存及稳定性
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile water to a concentration of = 0.2 mg/ml the antibody is stable for at least six weeks at 2-4ºC.
分析说明
Control
Human mast cells
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | mouse |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable neutralization: suitable western blot: suitable |
input | sample type hematopoietic stem cell(s) |
NCBI登记号 | NM_000899.3 NM_003994.4 |
UniProt登记号 | P21583 |
运输 | wet ice |
Gene Information | human ... KITLG(4254) |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich