产品说明
一般描述
Autophagy-related protein 13 (Atg13) is mapped to human chromosome 11p11.2. It comprises of HORMA domain (Hop1, Rev7 and MAD2).
特异性
Anti-Atg13 recognizes human, mouse, and rat Atg13.
应用
Anti-ATG13 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy.
生化/生理作用
Autophagy-related protein 13 (Atg13) is essential for autophagosome formation in mammalian cells. Atg13 forms a stable complex with ULK1 (Unc-51 Like Autophagy Activating Kinase 1) and FAK family-interacting protein of 200 kDa (FIP200). Mammalian target of rapamycin (mTOR) interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1, suggesting that mTOR regulates autophagy through the ULK1-Atg13-FIP200 complex.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
Store at –20 ℃. For continuous use, store at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots at –20 ℃. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~65 kDa |
| species reactivity | mouse, human, rat |
| 浓度 | ~1.0 mg/mL |
| technique(s) | western blot: 1-2 μg/mL using whole extracts of human HeLa cells and 0.5-1 μg/mL using whole extracts of mouse 3T3 or rat PC12 cells. |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... KIAA0652(9776) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
