Anti-Calreticulin (405-417) Rabbit pAb

产品说明

一般描述

Protein A purified rabbit polyclonal antibody. Recognizes the ~63 kDa calreticulin protein.
Recognizes the ~63 kDa calreticulin protein in heat-shocked HeLa cells.
Anti-Calreticulin (405-417), rabbit polyclonal, recognizes the ~63 kDa calreticulin in heat-shocked HeLa cells. It is validated for WB, ICC, IHC, and IP.

免疫原

Human
a synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH

包装

50 μL in Plastic ampoule

应用

Immunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)

Immunocytochemistry (see comments)

Immunoprecipitation (see comments)

Immunohistochemistry (see comments)

警告

Toxicity: Standard Handling (A)

外形

In PBS, 50% glycerol, pH 7.2.

重悬

Following initial thaw, aliquot and freeze (-20°C).

其他说明

Does not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Janicki, S., et al. 1997. J. Cell Biol. 139, 485.
Wada, I., et al. 1997. EMBO J. 16, 5420.
Nauseef, W.F., et al. 1995. J. Biol. Chem.270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息